Validación del método de cromatografía de gases para la determinación de Cannabinoides en muestras incautadas que ingresaron en el laboratorio de Química Forense del Departamento de Criminalistica de la provincia de Chimborazo.

Abstract

This research was conducted at the Forensic Laboratory of the Province of Chimborazo in order to validate the method of gas chromatography to determine Cannabinoids (Cannabis compounds) providing a number of tests which guaranteed reproducibility and reliability of results. First, an internal standard (tetracosane) mother of pure and used standard solution was analyzed and the following parameters: Selectivity resolution (RS) was analyzed by calculating getting values ​​above 1.5, the linearity was determined by the variation coefficient is r2 ≥ 0.999 r and t-Student with n-2 degrees of freedom and two-tailed α = 0.05 accepting H1 confirming that there is a linear correlation between variables, sensitivity was calculated from the slope, LOQ was 1,87378x10-9, performance and error 99.9994% 0.0006%, accuracy was obtained by the coefficient of variance (% CV) and Fisher's test was applied with 1 degree of freedom a queue for the numerator and 6 degrees of freedom for the denominator and α = 0.05, Ho is accepted demonstrating that there is significant variance in the data, to determine the robustness of a system test Youden and Steiner was applied. The analysis results showed that the method is suitable for the determination of cannabinoids in addition to the equipment variables such as temperature and the injection volume should not be modified by altering the results of significatival way.