Evaluación de la curva de crecimiento, cuatro sustratos, dos métodos de esporulación, tres portadores inertes para Trichoderma spp. y su comportamiento frente a ocho fungicidas y cuatro hongos patógenos en laboratorio.

Abstract

As an alternative method in combating diseases caused by soil pathogens and phyllosphere, it was considered to Trichoderma spp for its many qualities that have made him the ideal candidate in the management of agro-ecosystems. However, to be readily available and accepted, it is necessary to have appropriate formulations, so this research aimed at the determination of the growth curve and sporulation of Trichoderma spp .. A Complete random design was used with factorial arrangement with three repetitions, for mean separation Tukey 5% was used. The variables studied were: Growth curve of Trichoderma spp, resistance Trichodenna spp.. against Botrytis cmerea, Fusarium spp., Sclerotinia sclerotiorum and Monilla roreri., production of spores of Trichoderma spp from liquid culture incubated for 24, 48 Y72 hours, production of spores of Trichoderma spp in four solid substrates, Viability of spores Trichoderma spp three inert carriers. In order to know the best time to harvest conidia, the growth curve and sporulation of this fungus, the same as was determined between the fifth and sixth day was studied. However, Trichoderma viride and pseudokoningii stands for controlling Botrytis cinerea, while Trichoderma harzianum makes better control Monilia roreri where parasitism was observed. The best liquid medium was molasses (MI) incubated for 48 hours. For the production of spores of Trichoderma spp on solid substrates, treatments corresponding to amaranth and wheat in trays with or without mesh were the best, most spore production from molasses (MI) incubated for 48 hours, but the qualities amaranth physical make it the best solid substrate. Finally, although not statistically significant differences the three inert carriers, Selita should be chosen for wettable powder formulations and kaolinite for granular formulations for their physical qualities.