Actividad Antiinflamatoria y Citotóxica IN VITRO de las fracciones aisladas por Cromatografía Preparativa en Capa Fina de (Parmelina tiliacea)

Abstract

It was tested The anti - inflamatory and cyto - toxic activity of atranorin pulvinic dilactone compounds, isolated from Parmelina tiliacea lichen in order to study new molecules able to efflux contribute to anti - inflamatory effects without showing harmul side effects to human body, Parmelina tiliacea lichen was collected in San Miguel de Bolivar from Ecuador; at S 01ª 43.863’ W 079 ª 0.3069’ coordina tes and an Altitude of 2728 meters above sea level. The ethanol extract was obtained by means of reflux, from which, ATRANORIN, PULVINIC DILACTONE compounds as well by CRYPTOSTICTIC acid; were obtained by isolation in thin layer Chromatography (TLC) using toluene - ethyl acetate - formic acid as mobile phase , then, the identification of these compounds was carried out by means of Chromatographic and chemical tests; as well as by UV and IR spectroscopic comparision. In vitro test were conducted from atranorin P ulvinic dilactone compounds in neutrophils, isolated at a concentration of 1x10 7 N/mL by means of colorimetric assays based on tetrazolium salts. The outcomes of the test show that anti - inflamatory atranorina shows an inhibition of 8,3% lower; compared to aspirin and a difference of inhibition of 10,08% less than Pulvinic dilactone ; regarding to aspirin. In the test of cell cytotoxicity in vitro ; Pulvinic dilactone CL50 was 43.92 ( μ g/mL ) an the CL50 of the Atranorin was 44.22 ( μ g/mL ) . However, at 200ppm, th e difference shows 15.04% (cell viability) or 84.96% (cyto - toxcity) for atranorina and 20.47% (cell viability) or 79.53 % (cyto - toxcity) by applying this metod, the problem of inappropriate use of experimental animals; can be solved since it opens doors fo r new in vitro studies without having to use them.