Estudio Fitoquímico y evaluación de la actividad fotoprotectora IN-VITRO del componente Flavónico presente en Bidens Andicola (Ñachag)

Abstract

Was made a phytochemical study and the evaluation of the ph otoresist in - vitro activity of flavone component in Bidens andicola (Ñachag), developed in Natural products and Microbiology Laboratories, of the Science Faculty of the Polyt e c hnic School of Chimborazo in order to check w h ether the flavone metabolites can be applied to prevent negative effects by prolonged exposures to the sunlight as premature photoaging, eye disease, erythema and dermal carcinoma. A sample of the plant species was collected Bidens andicola (Ñachag) in I zamba Town, Tungurahua Province. Aft er the phytochemical study was conducted by organoleptic analysis, screening and removal of the fraction flavonic solvent with different polarities. From previously studies from Bidens andicola (Ñachag) plant presents flavonoids with structure correspondin g to derivatives of quercetin, w h ich was compared to a standard qualitative and quantitatively by UV spectrophotometry (UV - Vis) and infrared (IR). Then, an evaluation of phot oprotective activity was performed in - vitro using the method of cell death induce d by UV - A radiation in a pure strain of Escherichia coli . The sun protection factor was determined by the equation of Mansur and the median lethal concentration (CL50) of flavonic component. In the presence of phytochemical sc reening was the presence of fa tt y comp o unds, fla vo noids, tannins and anthocyanins. Was obtained the best extraction flavone in ethyl acetate. Compared with standard of quercetin there are compoun d s corresponding to the metabolite in a concentration of 0.67%. The evaluation of the activ ity photoresist in - vitro was determined that the flavone component extracted in ethyl acetate at a concentration of 100ppm has an effective activity of cytoprotection against UV - A radiation. The component flavone 100ppm has a sun protection factor of 20 an d the median lethal concentration (CL50) is 204,48ppm. Therefore it is concluded that the flavone component Bidens andicola (Ñachag) is a potencial agent photoresist in - vitro and is recommended effect the in - vivo studies for inclusion in formulations cosme ceuticals products to industrially level bases on natural ingredients for the prevention of skin cancer and other skin conditions from exposure to sunlight.