Estudio Fitoquìmico de Espino Chivo (Duranta triacantha)

Abstract

Is was realized the " Phytochemical s tudy of Espino chivo ( Duranta triacantha )", with the objetive of extract, purify and identify secondary metabolites , using chromatographic methods ; the vegetal specie collected in Guano canton , it was prepared for maceration in ethanol , the physical properties were conce ntrated and prepared, resulting a liquid greenish and sweet ex tract, with density 0.985g / mL , pH = 5.89 and refractive index 1.362 and for colorimetric tests are presented the functional groups of sesquiterpenolactones , alcaloids, benzoquinones and tanines. For the chromatographic purification was prepared t he cloroformic substrates and the hexanic sustrates,before to realize cromatographies in column, microcolumn , preparatory plates with cutting of bands , afterwards it is c oncentred and proved in th in layer chromatography, in system of proper solvents with evidence of effectiveness, efficiency and resolution , it was determined its spectrum ultraviolet ; isolating : the 1,3,4,7 - tetrahidroxi - 9,10 - Anthraquinone with Rf = 0.95 and λ max = 282.0nm , 292.0nm , 3 15.0nm , with a Rf of 0.44 whose spectrum UV reveal ed the λ max = 222.0nm , 276.0 nm , the flavone 1'5' - oxi - 7 - h9idroxiflavone, with Rf 0.76 with λ max = 212.0nm , 289.0nm , the 7 - hidroxi - flavananol and in the quantification for permanganometry was obtained 0.31 mEqg of picatecolics tanin e s like consumption of potassium permanganate. This phytochemical study e stablished the presence of flavonoids and tanines whose antioxidant, anti - inflammatory, antiseptic properties, benefit to the skin, besides contributes with studies about the plant and for its commercial use in the pharmacology and cosmetic industry in our country , according to the content of metabolites in the plant.