Evaluación de la Actividad Cicatrizante de un Gel elaborado a base de los extractos de Guarango (Caesalpinia Spinosa), Nogal (Juglans Regia) y Tomillo (Thymus Vulgaris) en ratones (Mus Musculus)

Abstract

The healing activity of a gel elaborated based on the extracts from guarango ( Caesalpinia spinosa ), walnut ( Juglans regia ) and thyme ( Thymus vulgaris ) with m inor injuries induced in experimental animals (Mice: Mus musculus ) were evaluated. It was used the extract of three plant species to prepare the gel, which was performed in the proper determination of the physical, chemical - qualitative parameters of qual ity (screening phytochemical), chromatographies, determining the types of excipients and the suitable quantities of extract for the healing gel preparation and its full quality control. It was experimented in 5 research groups: A (negative control), B (pos itive control: Lamoderm), C ( F l : Guarango 20%, Walnut 5% and Thyme 5%), D (F2: Guarango 15%, Nogal 10% and Thyme 5%) and E (F3: Guarango 10%, Walnut 10% and Thyme 10%), being these providing by mouth twice a day and during 12 days; and for Histopathologica l study was required removal the skin of the animal in this research. Gel presented a suitable pH of 6.31 compared to quality control; its characteristics are acceptable with total absence of contaminating micro organisms. The optimal formulations resultin g are F2 and F3 with a 100% in skin healing after Histopathological study. It was concluded that the gel has healing activity in minor skin wounds because of the secondary metabolites that possess some plants such as: phenolic compounds and/or tannins, fla vonoids and mucilages, metabolites that are responsible for the healing activity, perfect for the formulation of a pharmaceutical dosage form.