Identificación y cuantificación de Flavonoides en los extractos etanólicos de (Tagetes sp), (Tagetes multiflora) y (Tagetes zipaquirensis) por métodos cromatográficos

Abstract

The identification and quantification of flavonoids in the ethanolic extracts of Tagetes sp. (Tagetes multiflora) and (Tagetes zipaquirensis) were investigated by chromatographic methods, in the laboratory of HPLC and instruments of the Faculty of Sciences of the Polytechnic School of Chimborazo , With the purpose of creating natural insecticides that benefit the plants and improve the quality of them. Taxonomic identification, quality control of Tagetes sp, Tagetes multiflora and Tagetes zipaquirensis as raw vegetables and ethanolic extracts, physical - chemical tests were carried out, applying qualitative methodologies such as phytochemical screening and thin layer chromatography, observing The presence of three Rfs in the Tagetes sp belonging to the flavonoids isoramnetin glucosides with an Rf 0.50 and the quercetin Rf 0.60 and 0.68 in the Tagetes multiflora flavonoides type isoramnetine glycosides with Rf 0.13, 0.20, 0.25, 0.35 and Routine with the Rf 0.58 and for Tagetes zipaquirensis flavonoids of routine type with a Rf 0.51, chlorfenic acid with Rf 0.64 and phenolic acid Rf 0.74, for the quantification were applied the modern spectrophotometric techniques resulting in Tagetes sp with 11.03 ppm, Tagetes multiflora 17.36 ppm And Tagetes zipaquirensis 4.81 ppm and high performance liquid chromatography HPLC using as the mobile phase Ethane - Acetonitrile (75:25 v / v) and asserting pure concentrations in Tagetes sp with 31.04 ppm, Tagetes multiflora with 136.94 ppm and Tagetes zipaquirensis with 3.21 ppm. It is concluded that the best quantification method for the flavoniodes in the mobile phase extract Ethanol - Acetonitrile Tagetes sp, Tagetes multiflora and Tagetes zipaquirensis is HPLC allowing to obtain high concentrations of active principle. It is recommended to prepare a phytopharmaceutical applied to the industrial field as an agrochemical product, due to its high content of flavonoids and the insecticidal activity that has been investigated.