Evaluación del efecto ansiolítico del extracto Hidroalcohólico de flor de Badea (Passiflora Quadrangularis) En Ratones (Mus Musculus)

Abstract

Assessing the anxiolytic effect of the hydroalcoholic extract badea flower (Passiflora quadrangularis) in mice (Mus musculus) was performed; study in the Natural Products Laboratory, Instrumental Analysis and Bioterio Polytechnic School of Chimborazo to help reduce anxiety in patients with anxiety disorders, helping to improve their quality of life. With flowers badea Shushufindi collected in the province of Sucumbios, a hydroalcoholic extract quality parameters was developed, compounds were identified by phytochemical screening, thin layer chromatography (TLC) and UV-visible spectrophotometry. To effect a preclinical study was conducted using 18 mice Mus musculus divided into 6 lots of 3 mice each called G1 (White), G2 (positive control chemical), G3 (positive control natural) G4, G5 and G6 (Experiments Net). Such treatments, the G1 group received vehicle (saline: glycerol), zopiclone G2, G3 Passiflora incarnata, and G4 groups, G5 and G6 Passiflora quadrangularis extract 100%, 65% and 30% respectively. Each mouse was administered 0.2ml of oral treatment and after 30 minutes was performed 5 neuropharmacological tests (Test Wire, Burial of marbles, elevated zero maze, light-dark box and open field) that were used to assess the behavior of the Nervous System. Preclinical study results were analyzed by ANOVA and post-test statistic statistical HSD Tukey 95% confidence. Showing reduced anxiety levels in vivo in all three concentrations of Passiflora quadrangularis administered proving more effective concentration 100%. It is concluded that the flower extract possesses potential anxiolytic badea being alkaloids and flavonoids compounds responsible for this activity. It is recommended that this study be of therapeutic interest to develop new products and treat patients with anxiety disorders.