Evaluación del efecto antidiarreíco y cicatrizante de la infusión y del extracto etanólico de Cyclospermun leptophyllum (Pers.) Sprague en ratones (Mus musculus) y conejos (Oryctologus cuniculus)

Abstract

The evaluation of the antidiarrheal and healing effect of the infusion and ethanolic extract of Cyclospermum leptophyllum in mice (Mus musculus) and rabbits (Oryctologus cuniculus) was carried out at the Biotherium of the Escuela Superior Politécnica de Chimborazo. By means of the Experimental method, trials were carried out for quality control of the vegetable and ethanolic extract, evaluating the healing activity in incised wounds until their total healing, considering length, width and healing time in rabbits (Oryctolagus cuniculus,); the antidiarrheal activity was evaluated in mice (Mus musculus) induced with castor oil, considering frequency, type and grams of feces during 6 hours; we also studied the subacute toxicity; the results were analyzed by means of the Anova and Tukey tests with 95% confidence interval. The results of the physicochemical analysis showed that the ethanolic extract has pH 5.45, relative density 0.49g/ml, refractive index 1.35 and total solids 20.97%; the phytochemical screening showed high evidence of saponins, flavonoids, tannins, with less evidence of catechins, anthocyanidins, essential oils and sugars. Regarding the cycloaddition activity, it was concluded that it is effective, since it presents less healing time and less scar length. In the antidiarrheal activity, it showed efficacy when administered as a 5% infusion in water and ineffectiveness at 10%; the toxicological analysis indicated that Cyclospermum leptophyllum does not produce subacute toxicity. It is recommended to both students and teachers to carry out cicatrizant studies of the extract, using vehicles similar to the positive control to reduce errors; and to identify exactly the secondary metabolites present.