Evaluación de la actividad antiinflamatoria de extractos de Santa María (Piper peltatum) mediante el test de edema inducido en ratas (Rattus novergicus)

Abstract

To evaluate the anti-inflammatory activity of Santa Maria (Piper peltatum) extracts through the induced edema test in rats (Rattus novergicus), according to Winter was performed in the Biotherium of the School of Biochemistry and Pharmacy of the Escuela Superior Politécnica de Chimborazo. By means of the Analytical-Experimental research method, to evaluate if the extract of Santa Maria presents anti-inflammatory activity applying doses of extracts at different polarity with the purpose of finding the most adequate one. We used 28 rats divided into 8 groups: GAP (Randomized Positive Positive Group naproxen sodium), GAN (Randomized Negative Group carrageenan 1%), treated groups were: GAE1 (Experimental group 1 ethereal extract 2mg/kg), GAE2 (Experimental group 2 ethanolic extract 2mg/kg), GAE3 (Experimental group 3 aqueous extract 2mg/kg), GAE4 (Experimental group 4 ethanolic extract 1mg/kg), GAE5 (Experimental group 5 ethanolic extract 3mg/kg), administered orally, as vehicle 1% Tween 80 and distilled water. The volumes of inflammation were measured from 0 to 6 hours, for data analysis ANOVA test (analysis of variance) was used, and Tucker HSD 95% confidence. The anti-inflammatory activity was tested and the most effective dose was 3mg/kg. The toxicological and histopathological study of administered doses proved to be non-toxic and safe on the pharmacokinetic organs involved (Stomach, Liver, Kidney). It is concluded that this extract of Santa Maria showed an anti-inflammatory effectiveness, being the secondary metabolites flavonoids involved in such pharmacological capacity. I recommend that based on this research, a phytopharmaceutical that is feasible for its application should be elaborated.