Evaluación de la actividad diurética y cuantificación de polifenoles de Jamaica (Hibiscus sabdariffa L.) cultivada en Pomona, Pastaza - Ecuador

Abstract

The evaluation of the diuretic activity and quantification of polyphenols of Jamaica (Hibiscus sabdariffa L.) was carried out in the laboratories of Phytochemistry, Biotherium, and Instruments of the Faculty of Sciences of the ESPOCH using the calyxes of the flower for the diuretic effect in Rattus novergicus with average weights of 250 g treated in metabolic cages, by means of the experimental method. The objective of this work was to know the diuretic effect of the hydroalcoholic extract of the calyxes of Hibiscus sabdariffa L. administered via oro pharyngeal route, in 6 batches of rats representing the groups: White, Positive control and the different treatments RA, RB, RC, RD with different concentrations of phenols. The quantification of polyphenols by Folin-Ciocalteu method gave 0.29%. The identification of possible metabolites was performed by thin layer chromatography and column, with the solvent Ethyl acetate - 2-propanol - water - formic acid (6:2:2:2:1 v/v.) determining the presence of delphinidin-3-glucoside-xylose (Hibiscin) and cyanidin-3-glucoside-xylose. The diuresis of the Blank is 2 mL, the positive control (furosemide) gives 8 mL, the polyphenols 10, 20, 30, 40, 80 mg/Kg give 1.6; 3.5; 2.9; 1.9; 0.9 mL of urine respectively. It was concluded that with 20mg/Kg it has a percentage of diuresis in relation to the target of 75% and compared to the control of 47%, with a volume of excretion when administered of 78% and that the extract does not present any toxicity. It is recommended to continue with the study to verify the mechanism of action of the hydroalcoholic extract of Jamaican calyxes in its diuretic action.