Comprobación del Efecto Cicatrizante de una Crema a Base de Romero (Rosmarinus officinales), Matico (Pier aduncum) y Cola de Caballo (Equisetum arvense) en Heridas Inducidas en Ratones

Abstract

Verification of the healing effect of cream based on hydroalcoholic extracts of Rosemary (Rosmarinusofficinalis), Matico (Piperaduncum) and Horsetail (Equisetum arvense) on mice (Mus musculus) in the biotherium of the Faculty of Sciences of ESPOCH. The healing activity was evaluated through the induction of a wound in the scapular region of 15 previously shaved mice, 2 cm long by 2 mm deep, made with a scalpel, for the subsequent application of 5 treatments, being these: Control (+) = Treated with Procicar Cream, Control (-) = whites, Groups A ratio of 50:30:20 Group B ratio 30:50:20, Group C ratio 20:30:50 (Dosages) = Treated with the cream of fluid extracts of Rosemary, Matico and Horsetail, administered topically by 2 applications per day for 15 days. The results were subjected to a statistical analysis, for which ANOVA, T-student, 95% confidence interval tests were applied, obtaining an effectiveness of 67. 7% in Group C and 42% in Group A and B. It was concluded that Group C cream with a proportion of (20:30:50) has effective healing activity within 10 days due to the presence of flavonoids in the three plants and tannins in the horsetail that when combined improve the activity; the other treatments act as antibacterial taking 12 days to close the wound completely, all when applied topically do not present adverse effects at cutaneous level. It is recommended to perform more specific stability tests for commercialization of this cream based on the hydroalcoholic extracts of Rosemary (Rosmarinusofficinalis), Matico (Piperaduncum) and Horsetail (Equisetum arvense) due to their proven effectiveness.