Separación de Metabolitos Secundarios de Martín Galvis (Senna multifuga) con actividad antibacteriana

Abstract

Separation of secondary metabolites of Martin Galvis (Senna multijuga with antibacterial activity" aims to extract, purify and possible identification of secondary metabolites in the ethanolic extract and sub extracts this study was conducted in the laboratories of Phytochemistry of the Faculty of Sciences of the Polytechnic School of Chimborazo. The ethanolic extract shows sennosides similar to Sen (Cassia angustifolia). The phytochemical screening indicated that (Senna multijuga) contains anthraquinones, triterpenes, steroids, monoterpenes, sesquiterpene lactones, flavonoids and coumarins. For the separation of these metabolites were used chromatographic methods such as: column chromatography, microcolumn and for the purification preparative plate performing band cutting to subsequently concentrate and check in thin layer chromatography run in solvent systems the most appropriate in which it can demonstrate the 3 chromatographic parameters: efficiency effectiveness, efficiency and resolution subsequently determined in the ultraviolet spectrophotometer. The sub extracts; (II) presents 2 compounds at certain wavelengths(?) max 1=226 nm (coumarin) 2=304 (flavonoid) using solvent of run: Toluene, CH3COOH (93:3. 7), and as developer KOH and EtOAc (100%) developed with CeSO4; the (IV) 2 compounds with wavelengths (?)max 1= 228 nm (ß sitosterol); 2= 276 nm (sesquiterpene lactones) using running solvent EtOAc, MeOH (95:0. 5); EtOAc; Toluene, EtOAc ;(80:2) and as developer solvent Rosentaler and (I) presented 2 compounds at (?)max 1=302 nm (chrysophanol) and 2=313nm using running solvent CH3COOH, EtOAc,1 propanol; (1:40:40) and developed with alcoholic KOH applying the Woodward-Fieser rules it is concluded that it is rehine. It is recommended to deepen this research of these secondary metabolites identified, to be applied in the field of Phytomedicine and Phytotherapy and thus contribute to the development.