Evaluación de la Actividad Antiinflamatoria de la Escorzonera (Perezia multiflora) en Ratas (Ratus novergicus)

Abstract

The present research aims to evaluate the anti-inflammatory activity of Scorzonera (Perezia multiflora) in rats (rattus novergicus), carried out in the phytochemistry laboratories of the Faculty of Sciences of the Escuela Superior Politécnica de Chimborazo. The anti-inflammatory activity of the extract and sub-extract is evaluated by the method of Wintter et al, by inducing edema in 15 rats (rattus novergicus) which were injected with carrageenan (0.5%) 0.1 ml in the subplantar region causing inflammation, for the subsequent application of the 5 treatments: T1, extract 92.07 mg/kg; T2, extract 46.03 mg/kg; T3, chlorophormic sub-extract of 5. 30mg/Kg ;T4, butanol sub-extract 11.77 mg/Kg and T5, ethereal sub-extract 2.32 mg/Kg,T6 ( positive control), treated with diclofenac sodium, T7 (negative control), received only the vehicle distilled water, oral administration with the use of syringes with needle adapted to be used as orogastric tube. The volume (length and diameter) of the paw was measured at 0, 1, 2, 2, 4, 6, 8, 10, 12 hours. With the data obtained, graphs were made, using the Microsoft office Excel 2007 program, in which it was observed that the maximum swelling was obtained at the eighth hour of administration, with a percentage of swelling for T1 (extract 92. 07 mg/kg) 19.23; T2 (extract 46.03 mg/kg) 73.16; T3 (chloroformic sub-extract 5.30 mg/kg) 107.9 ; T4 (butanolic sub-extract 11.77 mg/kg) 69.65; T5 (ethereal sub-extract 2.32 mg/kg) 138.71; T6 (diclofenac sodium) 16.45; T7 (negative control) 145.04. The concentration of 92.07 mg/kg presented an inhibition % of 50.8 and while Diclofenac sodium presented an inhibition % of 52.5 and for T4, T2, T3, T5 it was 30.5; 30.3; 15.4 and 3.8 respectively. The results were subjected to a statistical analysis, for which the analysis of variance was applied, with a significance level of 0.05. In the evaluation it was concluded that treatment T1 (extract 92.07 mg/kg) is effective, since it carries the totality of secondary metabolites and in higher concentration with anti-inflammatory properties such as phenolic compounds, flavonoids and steroids. It is recommended to elaborate a phytopharmaceutical with the dosage of T1(extract 92.07 mg/kg) to facilitate its storage and administration.