Comprobación del Efecto Laxantes del Extracto Etanólico de Raíces y Hojas de Taraxaco (Taraxacum officinale) en Ratones (Mus musculus)

Abstract

Verification of the laxative effect of the ethanolic extract of roots and leaves of taraxacum (Taraxacum officinale) in mice (Mus musculus)" in the Biotherium of the School of Biochemistry and Pharmacy, Faculty of Sciences, Escuela Superior Politécnica de Chimborazo from October 20 to November 24, 2012. An experimental method was carried out using taraxacum roots and leaves extract in three groups of three mice, each one denominated according to the concentration of the extract that was administered at 40%, 70% and 100% respectively, for which a cannula and three syringes were used. To evaluate the laxative activity, taraxacum extract was administered to the experimental animals at 40%, 70% and 100% for 5 days. The weight of the feces of the mice per day was quantified. The following results were obtained: Taraxacum 40% 0.159 g, taraxacum 70% 0.195 g and taraxacum 100% 0.257 g. These data were statistically analyzed by applying the G-Stat 2.0 program using the ANOVA method with one factor for multiple comparisons. Method: Tukey HSD at 95.00% sensitivity. It was demonstrated that the higher the concentration of the extract, the greater the laxative effect, that is to say that the 100% extract was more effective than the 40% and 70% concentrations. The 100% extract did not show any results in the acute toxicity test, so its administration is safe in animals. - 64 - It is recommended that an advanced study be carried out to extract inulin from taraxacum, which can be used for pharmaceutical formulations.