Separación y Posible Identificación de Metabolitos Secundarios de la Jacaranda (Jacaranda mimosifolia) con Fines de Aporte a una Técnica de Análisis Químico

Abstract

Jacaranda (Jacaranda mimosifolia) used as an antibacterial, this research is oriented to the chemical analysis technique for it is separated and identified secondary metabolites such as: jacaranone, jacoumaric acid and methyl jacaranone in the Laboratory of Phytochemistry, Fac. Sciences; it is prepared by maceration with ethanol, concentrated by evaporation and analyzed with staining and identification reactions for flavonoids (Shinoda), saponins (foam), terpenoids (Libermam Buchart), and lactones (Baljet). Ethanolic extract fragments in toluene, butanol and residual ethanol, GF254 silica gel stationary phase thin layer chromatography of the ethyl acetate sub extract run fraction 1 in chloroform: ethyl acetate (93:70) and revealed with Ce(SO4) gives an RfB4: 0.79 at ?max= 230, 279 nm inferring the structure of a coumarin. Thin layer chromatography of fraction 10 and 11 give an Rf : 0.5 with ?max = 203,300 nm possibly corresponding to jacaranone and methyl jacaranone in fraction 12 gives an Rf= 0.52 at a ?max = 203, 276 nm possibly corresponding to jacoumaric acid, run in Chloroform: Methanol (4:1), revealed with Vanillin H2SO4. To the butanolic subextract treated with ether, an ethereal sub-sub-extract is obtained, the chromatography in thin layer with chloroform:Methanol:Water (65:35:15) revealed with Ce(SO4), presents similar Rfs for what the sample is fractionated in chromatographic column from fraction 3 to 5 give round, separated and similar spots for what they are joined, chromatographed in AtOAc:Me(OH):Water (10:2. 4:1) for what a preparative plate is applied, in the verification of compounds it is had in the band 3 an Rf = 0.4 with ?max = - 85 - 203, 231, 279, 310 nm, in the band 4 an Rf = 0.66 with ?max = 202, 229, 279, 310 nm; determining possibly flavonoids and the structure of an Isoflavone. Verifying that up to two peaks correspond to terpenes and three to flavonoids.