Determinación de la Actividad Antimicrobiana del Extracto Etanólico de las Hojas y Flores de Iso (Dalea mitisii)

Abstract

In the laboratories of the School of Biochemistry and Pharmacy - ESPOCH. The determination of the antimicrobial activity of the ethanolic extract of the leaves and flowers of Iso (Dalea mutisii Kunth) was carried out through the application of the Mistcher test with the objective of contributing to the research of new phytopharmaceuticals that help to fight infections caused by pathogenic agents. The extract of Iso leaves and flowers was obtained by maceration; the raw material and the product underwent a rigorous quality control. Microbial inhibition was evaluated using the Mistcher method, which is based on the inhibition of the exponential growth of microorganisms susceptible to bioactive molecules diluted in the agar medium, against strains of Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 9637, Klebsiella pneumoniae ATCC 10031, Pseudomonas aeruginosa ATCC 27853, Salmonella Gallinarum ATCC 9184 and Candida albicans ATCC 10231; at concentrations of 10000, 1000 and 100 µg/mL; likewise, combinations of the total extracts were used at a ratio of 50:50: 50. Previously, a phytochemical study of the extracts was carried out in order to identify their chemical components. The total extract of leaves at 10000 µg/mL has antimicrobial activity against Staphylococcus aureus and partial activity at 1000 µg/mL. The flower extract at 10000 µg/mL has activity against Candida albicans. The combination of both extracts at 10000 µg/mL is active against Staphylococcus aureus and partially active against Candida albicans. It was concluded that both extracts including the mixture of both extracts show antimicrobial activity at concentrations of 10000 µg/mL against Staphylococcus aureus ATCC 6538 and Candida albicans ATCC 10231; possibly due to the abundant phenolic compounds contained in the extracts. It is recommended to carry out Iso studies based on their cytotoxicity; since they can be of great contribution in phytopharmaceuticals against cancer.