Evaluación de cuatro tratamientos pre-germinativos en Magnolia spp, A nivel de laboratorio, en la provincia de Chimborazo

Abstract

This research aimed to measure the germination percentage of the Magnolia pastazaensis species identified and described through a dendrological sample currently located in the ESPOCH Herbarium. The experimental work was carried out in the Faculty of Natural Resources seed laboratory. The biological material (seeds) was transferred from the Triunfo Puyo-Pastaza parish, in which the tests of the ISTA standards were first applied, giving the following results: by weight 171.36 g, there are 238 seeds, 100% in terms of purity and humidity, an average of 30.37%. This last test was carried out by drying the seeds in an oven for 24 hours taking data before and after this procedure. Additionally, to check the germination percentage, three trees were used and four pre-germination treatments were applied: P1: Control; P2: Self-tapping water immersion for 24 hours; P3: Inhibition in AG3 with 50 mg/L and P4: Temperature change (22 days in refrigeration), applying the wholly randomized experimental design with 12 treatments in total, which were first placed in a culture medium where contamination was evident, so it was moved to the promix, resulting in the germination of the only plant in the trial after 55 days, belonging to T5 (Tree 2 + control pre-germinative treatment), dying 70 days after germination without having developed its roots, leaves and with a height of 13 cm. Therefore, it is concluded that the germination percentage was relatively low since only one 180 seed sprouted due to the contamination generated by the sarcotesta that contained a large amount of oils, rotting the seed embryo over time. Therefore, no treatment had a positive effect on germination.