Evaluación de tres curvas de congelación para la criopreservación de semen equino en el complejo intercultural y deportivo Mushuc Runa

Abstract

The objective of this work was to evaluate three freezing curves for the cryopreservation of equine semen at Mushuc Runa Intercultural and Sports Complex, located on Ambato - Riobamba road, at Santa Lucia Neighborhood, in Tisaleo Canton, Tungurahua Province with a duration time of 120 days. For the development of the present investigation, 108 experimental units were evaluated, which corresponded to the seminal doses (thawed straws) obtained from the semen extraction of 2 equine stallions of the Spanish and Friesian breeds, which were collected 3 times with an interval of 8 days. The experimental units (straws) were distributed under a Completely Randomized Design (CRD) with a combinatorial arrangement of factors, where factor A is the freezing curves and factor B is the races of quinoa. It was determined that the characteristics of fresh equine semen are similar in both stallions; however, a higher ejaculate volume is reported in the Friesian quino with 130.0 ml in relation to that of the Spanish breed with 66.67 ml. It was concluded that according to the freezing curves the microscopic characteristics show higher values in individual motility and sperm viability at 2 and 3 hours of freezing. It was recommended to use three hours within the equine semen freezing protocol because it presented higher levels of individual motility, membrane integrity and sperm viability (%).