Efecto de los plaguicidas en la microbiota y en la respiración del suelo en la comunidad Utuñag del cantón Penipe provincia de Chimborazo

Abstract

The objective of this research was to determine the effects of pesticides on the microbiota and soil respiration in the community of Utuñag in Penipe canton, Chimborazo province. Soil samples were collected according to the phenological cycle of the potato until the flowering period. The first sample was taken from the soil without any disturbance and the other 3 samples were applied with fungicides. For the samples that were taken to the soil laboratory, microbial respiration was performed using the Basal Method, which is performed by determining the production of O2 in the medium or the concentration of CO2 released. Regarding the determination of the colony-forming units of bacteria and fungi, soil samples were prepared from which bacteria and fungi were isolated from both mother solutions and 10−1, 10−2, 10−3 dilutions, which were spread in petri dishes with Papa Destroxa Agar (PDA) and Nutrient Agar (AN) medium, the number of Colony Forming Units (CFU) was recorded. Regarding the cultural characterization, the edge, shape, surface, front color and back color of the colony were recorded. With the results obtained from the cultural characterization, the genus of the microorganisms was identified, obtaining a total of 9 species of fungi and 11 types of bacteria.