Diseño de una metodología en propagación in vitro de Prunus serotina spp capulí Ehrh, a partir de segmentos nodales de árboles élite para el aprovechamiento sostenible del patrimonio natural en la provincia de Tungurahua

Abstract

The objective was to design a methodology for the in vitro propagation of Prunus serotina ssp. capulí Ehrh from nodal segments of elite trees. The methodology involved a quantitative and experimental approach, using disinfection and in vitro culture techniques, and evaluating variables such as shoot number and length. The results showed that a 10-minute treatment was optimal for sprouting and phenolization, with significant variability in in vitro sprouting efficiency between nodal segments of different elite trees. Phase 2 of multiplication was characterized using treatments to optimize the number and length of Prunus serotina shoots in 30 days, with treatment 4 standing out as the most effective. However, the impossibility of completing phase 3 of rooting due to time constraints left a crucial part of the study incomplete. In conclusion, the results indicate that a 10-minute treatment is optimal for propagating Prunus serotina ssp. capulí Ehrh, balancing contamination reduction and improved viability, budding and phenolization, but the failure to complete the rooting phase underscores the need for future research to fill this critical gap.