Determinación del daño en el adn espermático de semen porcino diluido con diferentes niveles de agua de coco

Abstract

The present research work was carried out at the Swine Genetics Center “LICAN GENETICS” located in Licán Parish, Riobamba Canton, Chimborazo Province, with the objetive of determining the damage in the sperm DNA of swine semen diluted with different levels of coconut water. Ninety-six samples of Hampshire and belgian White swine semen were used under a completely randomized tri factorial design. The results obtained when evaluating the characteristics of swine semen diluted with different levels of coconut water substitutio, showedthe highestvalues of individual progressive motility (80.52%), sperm viability (81.15%), and sperm membrane integrity (83.02%), when using 25% coconut water substiyuyion. In addition, it was establised that the best post dilution time was 24 hours. On the other hand, the macroscopic characteristics evaluated show that the semen of the swines has a normal appearance, a creamy White color, a neutral smell and a pH of 7.00 indicating that there is no abnormality. It is concluded that the use of coconut water as a natutal extender does not negatively influence the motility, viability, sperm membrane integrity and chromatin integrity of the two evaluated swine breeds. It is recommended to maintain good semen handling and care practices to ensure adequate individual motility and improve fertility rates in artificial insemination in swine, since semen with good mass motility increases the chances of successful reproduction.