Efecto de un antimicrobiano elaborado con Acetobacter aceti aislados del bosque primario-Pungalá en la empresa Arsaico

Abstract

This research aims to explore the utilization of biotechnologically significant microorganisms for surface disinfection, specifically stainless-steel tables. The primary objective was to develop an organic antimicrobial agent using Acetobacter aceti isolated from the Pungalá Primary Forest, compared with a commercial antimicrobial. The methodology employed a Completely Randomized Design under a linear model with four treatments, each replicated four times. Descriptive statistical procedures were applied for physicochemical and microbiological analyses pre- and post-application, employing ADEVA and Turkey's test (p<0.05). Physicochemical tests of the treatments revealed statistical differences in pH, total acidity, and volatile acidity, albeit within the limits set by the INEN 2296 standard. Microbiological values obtained pre-application indicated the presence of Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Salmonella spp., exceeding the maximum limits set by UNE-EN ISO 18593 and Ministerial Resolution No. 461. Post-application microbiological results for Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Salmonella spp showed the absence of these pathogens in treatments T0 and T3, conforming to UNE-EN ISO 1893 and Ministerial Resolution No. 461, while treatments T1 and T2 determined a minimal reduction in microbial population. Statistical analysis revealed significant differences among treatment means, with treatment T0 displaying higher values for pH (2.51), total acidity (5.13%), and volatile acidity (5.11%) pre-application. Following application, treatments T0 and T3 demonstrated the absence of the identified pathogenic microorganisms on stainless steel tables.