Biorremediación de muestras de suelo contaminado con hidrocarburos totales de petróleo (tph).

Abstract

One of the most worrying environmental pollution problems is release of total petroleum hydrocarbons into the soil, air, and water; then, these have been causing great natural disasters with their toxicity. In the present research was evaluated the efficiency Pseudomona fluorescens to biodegrade soils hydrocarbon contaminated with aim of reducing TPH (Total Petroleum Hydrocarbons) on soil. This project addressed two phases: the field phase consisted of soil sampling and laboratory phase: consisted of the installation from trial with a 3x3 system consisting of a few treatments at three different concentrations of Pseudomona fluorescens and each treatment with its respective three replicates, controlled environmental conditions like: pH and electrical conductivity. Physical-chemical and TPH analyses were performed at the beginning end of trial every 15 days for two months. The initial TPH contamination on soil was 34792.82 mg/kg, and as a result it was obtained that T1 was the best treatment since it was the most efficient, being within the permissible limits of Ministerial Agreement 097-A; demonstrating that the treatment works independently, being T1 who degraded more amount of hydrocarbon reaching 607 mg/kg. The efficiency Pseudomona fluorescens bacteria to biodegrade TPH was demonstrated, using hydrocarbons as a carbon source, metabolizing them, and converting them into water and CO2, showing that bioremediation is an ecological and effective process.