Análisis antimicrobiano de los aceites de Artemisia absinthium y Borago officinalis frente a Escherichia coli Y Klebsiella pneumoniae

Abstract

The objective of this study was to evaluate the antimicrobial activity of Artemisia absinthium (wormwood) and Borago officinalis (borage) oils against Escherichia coli and Klebsiella pneumoniae. It had a quantitative approach and experimental design since there was a manipulation of the concentrations of the oils. To obtain the essential oils, the plants were first collected and conditioned, the extraction was made by the hydro distillation method, then quality control tests were performed such as density, refractive index, yield percentage, physicochemical and organoleptic characteristics; to determine the in vitro antimicrobial effect it was made by the disc diffusion method; while for the determination of the MIC and CMB presented by the strains, it was made by the dilution method. The results showed that the antimicrobial effect of the essential oil of Artemisia absinthium and Borago officinalis at a concentration of 5000 ppm presented inhibition halos against the two strains; as for the MIC the Borago officinalis oil was 50 μL/mL and the Artemisia absinthium oil was 25 μL/mL against both strains, on the other hand the Borago officinalis oil presented a CMB of 25 μL/mL against the two strains and the Artemisia absinthium oil presented a CMB of 50 μL/mL in both cases. In conclusion, the essential oils obtained meet the quality parameters, according to the Duraffourd scale, it could be determined that both oils are sensitive against the two strains. Further studies are recommended in order to identify which are the active principles contained in the oils to produce this effect and to test the effect of these oils against gram-positive strains.