Síntesis verde de nanopartículas de hierro a partir de Piper aduncum (MATICO) para evaluar su actividad bactericida en muestras del río Chibunga

Abstract

The synthesis of nanoparticles is being of great relevance in the field of biomedicine and has a variety of applications, including the use of nanoparticles for water treatment, which is currently considered a source of harboring potentially pathogenic bacteria that are acquiring resistance to antibiotics. The present study had the objective of obtaining iron nanoparticles by green synthesis to be evaluated as bactericidal agents in water samples from Chibunga river. The iron nanoparticles were synthesized using a rapid, one-step and completely ecological method that employs aqueous extract of Piper aduncum (matíco) leaves as reducing and protective agent, the obtained nanoparticles were characterized by Scanning Electron Microscopy (SEM) and Ultraviolet Visible Spectroscopy (UV- vis). As a result, nanoparticles with an average size between 15 - 24 nm were obtained. Two areas of the sample were compared and the presence of iron nanoparticles in aggregate form was verified, since, at first sight, white clusters appear on the surface of the sample. Similarly, the antimicrobial activity of the nanoparticles was done by means of bacterial inhibition pre-tests in solid culture media (nutrient agar), determining the growth of the bacteria. For the analysis of the experimental design, Pearson's correlation coefficient was used to establish whether or not there is a relationship between the variables time and growth. It is concluded that the aqueous extract of the matíco plant is a suitable reducing agent for the synthesis of iron nanoparticles due to its good stability in solution and the UV- Vis wavelengths it presents. In addition, they present a sustainable alternative for the treatment of water samples from the Chibunga river.