Estudio del efecto sinérgico de la combinación de amoxicilina y ciprofloxacina frente a klebsiella pneumoniae resistente a antibióticos betalactámicos

Abstract

The objective of this research was to study the synergistic effect of the combination of amoxicillin and ciprofloxacin against Klebsiella pneumoniae resistant to beta-lactam antibiotics: the minimum inhibitory concentration (MIC) of the combination of amoxicillin and ciprofloxacin was determined against a strain of Klebsiella pneumoniae resistant to beta-lactam antibiotics, the efficacy of the synergy of the combined antibiotics was compared with the concentration of the antibiotics individually, and the effectiveness of the combination of amoxicillin and ciprofloxacin with a last- line antibiotic such as tetracycline was tested. The results showed that the minimum inhibitory concentration (MIC) of the antibiotics was 64 µg/mL for amoxicillin and 32 µg/mL for ciprofloxacin. In order to evaluate the synergy of the combination of the antimicrobials, the fractional inhibitory concentration (FIC) index was calculated, which was 0.5, proving the synergy between the antibiotics. When comparing the efficacy against Klebsiella pneumoniae, it was determined that the MIC of tetracycline was 128µg/mL and the combination of antibiotics resulted in a reduction of the MIC to 16 µg/mL for amoxicillin. and ciprofloxacin, since the effect against beta-lactam-resistant Klebsiella pneumoniae, which is one of the main bacteria causing infections in individuals, was potentiated. It is recommended to evaluate the in vitro synergistic effect of more antibiotic combinations as a proposal for the treatment of bacterial infections with resistant or multi-resistant etiology in hospital and community environments.