Evaluación de 3 protocolos de crioconservación de semen equino Anglo Arabe y Pura Sangre Inglés (psi) en la Estación Experimental Tunshi de la ESPOCH

Abstract

This research sought to evaluate three protocols for cryopreservation of equine semen in Anglo Arabian and English Thoroughbred breeds at the Tunshi Experimental Station of ESPOCH. The protocols varied in times and G-forces in the centrifugation prior to cryopreservation (15min - 2000 RPM; 20min - 1500 RPM; 25min-1000 RPM). Commercial diluents RAMP PLUS for centrifugation and CRIO RAMP for cryopreservation were used according to the manufacturer's recommendations. The evaluation included fresh and post-freezing semen characteristics to determine the efficiency of each treatment. Using a freezing curve, semen was cooled to 4 degrees Celsius for 4 hours and then frozen in liquid nitrogen vapor for 10 minutes. Ten experimental units per treatment and breed were evaluated, totaling 180 experimental units in three semen extractions. The results of fresh semen were analyzed with a randomized complete block design, assigning each block to the males used. For post-freezing semen, a completely randomized design with a combinatorial arrangement of factors was applied, with factor A being the times and G forces in the centrifugation and factor B the equine genetic line. The interpretation of results was performed with the statistical software InfoStat version 2021, using descriptive statistics analysis, ADEVA and separation of means. The best results were observed with T3 (25min - 1000 RPM), with an individual motility of 61.75% and sperm membrane integrity of 50.07%. It was concluded that the most effective protocol for equine semen cryopreservation is T3, discouraging T1 (15min - 2000 RPM) due to its lower results in motility and sperm membrane integrity.