Estudio de la Toxicidad Sub-crónica de la Nano Plata en Ratas Wistar

Abstract

The nano silver studies that are being carried out for the benefit of health do not have a scientific support that guarantees the safety in the use of this product in the long term, so the study of sub-chronic toxicity at 90 days in Wistar rats of both sexes of the Biotherium of the School of Biochemistry and Pharmacy of the ESPOCH was proposed. The methods used were those described by the OECD standards, randomly distributed in 5 groups: control group to which no substance was administered, control group to which water was administered as vehicle and 3 groups treated with the nano silver suspension in doses of 1, 2 and 4 mL of 25 ppm, which corresponds to a cumulative dose of 9, 18 and 36 mg/kg respectively. Toxic signs and body weight were determined weekly. Hematological indicators (hemoglobin, hematocrit, platelets, total leukocyte count and total erythrocyte count), blood chemistry (uric acid, albumin, cholesterol, creatinine, total proteins, ALAT, ASAT, alkaline phosphatase and gamma GT), histopathological examination of organs and tissues (liver, stomach and kidneys) were evaluated. The behavior of the animals, toxic signs, mortality were not affected by the administration of the nano silver suspension in any of the assays. The analyzed parameters of body weight, hematology, blood chemistry and histopathological analysis of organs and tissues did not show significant toxicity attributable to the test substance.