Evaluación de la actividad antibacteriana del extracto etanólico de Lonicera japonica (Madreselva) para la elaboración de una crema tópica

Abstract

The main objective of this research was to test the antibacterial activity of the ethanolic extract of the leaves of Lonicera japonica (honeysuckle) for the preparation of a topical cream. The methodology implemented had a quantitative approach of correlational scope with an experimental design; the study population was the leaves of honeysuckle collected by the simple random sampling method. The ethanolic extract was obtained by percolation; phytochemical screening and thin layer chromatography were performed to identify the secondary metabolites present; the antibacterial activity was evaluated by two methods: Kirby-Bauer and dilution in broth against ATCC type bacteria (American Type Culture Collection) Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa; the extracts were conveyed in a base cream and the quality control of the finished product was performed. By means of this methodology, it was established that this vegetable species contains flavonoids, chlorogenic acid among other components; the antibacterial activity was evidenced at the following concentrations: 64000, 8000, 250 and 125 µg/mL and when transmitted, it was determined that the minimum concentration at which bacterial inhibition existed was 125 µg/mL; the quality control of the final product was performed and it had the optimum organoleptic, physical-chemical and microbiological characteristics complying with the specifications required by the Ecuadorian Technical Standard INEN (Ecuadorian Standardization Service) 2867: 2015. It was concluded that Lonicera japonica has considerable anti-bacterial activity as it is a plant and that the formulated topical cream can be of great help for skin disinfection in order to avoid contamination of pathogenic bacteria through contact from one person to another.