Evaluación de la actividad fotoprotectora de una crema a base de Oreocallis grandiflora en la especie Cavia porcellus

Abstract

The objective of the project was to evaluate the photoprotective efficacy of a formulation based on the extract of Oreocallis grandiflora, through its in vivo application in the species Cavia porcellus. For which the collection of drying, grinding, maceration, and filtration of the plant material was carried out in order to concentrate phenolic compounds and achieve a more stable active principle. Once the dry extract was obtained, 5 topical formulations of the Beeler base type were prepared: placebo, octylmethoxycinnamate, with the active principle of O. grandiflora (0.1%, 0.2%, and 0.4%), the same ones that were applied directly to the skin of 25 previously shaved guinea pigs. For this, 5 group analyses were established, in each group, the guinea pigs were exposed to UV-B radiation for a period of 10 minutes (at 300 nanometers) and the erythema levels were recorded with a mexameter. The results for the erythemic difference analysis were: A) F1 (84, 51, 87, 72, 71); B) F2(0, 1, 1, 1, 1); C) F3(36, 35, 31, 26, 32); D) F4(12, 6, -8, 4, 4); E) F5 (0, 1, 6, 2, 0). For the analysis of the Sun Protection Factor (SPF) recordings were taken every 10 minutes during 4 consecutive rounds of radiation in the placebo group and the formulation with the highest performance (0.4%), obtaining the following results: Placebo (10 min: 11,39; 20 min: 18,21; 30 min: 25,66 y 40 min: 34,14), formulation of O. grandiflora 0,4% (10 min: 1,92; 20 min: 4,97; 30 min: 6,16 y 40 min: 8,28) and the SPF: 4. It was concluded that Oreocallis grandiflora has photoprotective activity, this quality is granted by its concentration of phenolic compounds, in addition, its efficiency was two times higher than the control formulation. Finally, time management was recommended in order to avoid causing harm to the guinea pig.