Evaluación de la actividad ansiolítica de la combinación de extractos de lavanda (Lavandula sp) y naranja (Citrus reticulata) en ratones (Mus musculus)

Abstract

The aim of this research was to determine the anxiolytic activity of the combination of lavender (Lavandula sp) and orange (Citrus reticulata) extracts in mice (Mus musculus). The study was quasi-experimental and prospective, the plant material was collected and the aqueous, ethereal, alcoholic and hydroalcoholic extracts were prepared by weighing 50g of dried and crushed plant material, and once the extracts were obtained, a phytochemical screening analysis was performed. To obtain the concentrations of 150 ppm, 200 ppm and 250 ppm, the extracts of the plant samples were lyophilized, likewise, to determine the anxiolytic activity, 5 study groups consisting of 6 mice were used, working with the blank group, the control group and with 3 different dose concentrations, the control group was administered clonazepam with 15% propylene glycol as vehicle, the blank was not administered any solution. In addition, the ANXIOLITIC LAB software was used for data processing in the different behavioral tests: light-dark field (LDF), elevated cross maze (ECM) and open-field (OF), on the other hand, the database was generated in EXCEL spreadsheet. The results of the phytochemical screening showed alkaloids and terpenes as main metabolites, with respect to the result of the anxiolytic effect of the different concentrations it was concluded that the third concentration has an anxiolytic activity similar to clonazepam. It is recommended that a comparative study be carried out with the same species individually.