Determinación de la Actividad Hipoglicemiante del Extracto Hidroalcoholico de Justicia chlorostachya Leonard (Insulina) en Ratones con Hiperglicemia .

Abstract

"This thesis deals with the determination of the hypoglycemie activity of ho vegetal insulin (Justicia chlorostachya Leonard in mice with induced hyperglycemia (because ofoverload of sucrose at 4 y / kg weight relationship). carried out at the Bioterio of th. Biochemistry and Pharmacy School of the ESPOCH.

With be application f the empiricamayical method, te biological material (3040 y tino mice weigh) vas divided imo 6 groups named RG: Negative contro, RG2: Posive Control (Acarbose 07mg/Kg), RG: Dose 1 (Je 16 mg/Kg), ROS: Dose 2 (62 mg of Je / kg, ROS: Dose 3 (64 mg of Je / kg), RS: Wines. Evening

Gilycacmia at 0, 30, 60 and 120 minas aftr sucrose overload, through reactivo strips sing GlucometerProdige.

Data obiined bis way were analyzed sttisically through ANOVA a factor with grouped data and the Tukey test HSD (Honesty Significant Difference) at 99% rliabiliy interval. The resul clearly show the hypogiycemie effet of he hydro alcohol extract of Justice clrostachya, tessing that from the lowest dosage 16 mg / kg weight it proved to be a substituting potential as efcient s the treatment wi acarbose (0.7mg/Kg wcigh) with 1x10-14 povalu, Moreover it was cvident that with th toxicologcal and hystopathological study the administred dosages proved to be atoxic, and histlogically safe over the pharmacokinctic organs involved (Stomach, Live, Kidney).

It is concluded that the cilnobotanic uso given to vegetal insulin, as a hypoglyeemic “gent, is sciemifially tests with the present investigation, and that the protagonistic secondary metabolites in such a pharmacological capacity ac phenolis compounds with avonoid prototype.

IK is Recommended from this scietfic work, to continue further investigations om Justicia chlorostahya, so as to contribute to improve the lifestyle of he diabetic patient."