Caracterización de microorganismos para uso agroindustrial aislados de un bosque primario en el canton Cumandá.

Abstract

This study aimed to characterize microorganisms for agroindustrial use isolated from a primary forest in the Cumandá canton and to analyze their physical-chemical properties. Biochemical tests and physical-chemical analysis of the soil identified the microorganisms. Sampling was carried out with the help of an auger. Then a stock solution was developed to make dilutions to seed the microorganisms in MRS, Acetobacter glucose, and Sabouraud agars, which were incubated for 48 hours at 37, 27, and 30º C. Subsequently, isolation was carried out to identify the microorganisms concerning their morphology (color, surface, shape, and gram staining). Biochemical tests such as catalase and oxidase for lactic and acetic acid bacteria and potassium hydroxide for LAB were then performed. Characterization tests such as carbohydrate fermentation and YPD broth for yeasts were also performed. Four species of lactic acid bacteria, two species of acetic acid bacteria, and yeasts were obtained, resulting in lactic acid bacteria positive for staining and negative for oxidase, catalase, and KOH; acetic acid bacteria were harmful to staining and oxidase but positive for catalase. The physical-chemical analyses were carried out according to the regulations established for each mineral. It was concluded that the species isolated might belong to the genus Lactobacillus, Lactococcus, Bifidobacterium, Acetobacter, and Saccharomyces. It is recommended to employ other biochemical tests such as API 50 CHL or real-time PCR for better identification of each of the isolated microorganisms