Evaluación de diferentes protocolos para el establecimiento in vitro de mora de castilla (Rubus glaucus Benth) a partir de segmentos nodales.

Abstract

This research aimed to evaluate the different protocols for the in vitro establishment of Castilla mulberry (Rubus glaucus Benth) from nodal segments, for which three treatments (protocols) were proposed with three replications consisting on T1 (Protocol 1): 70% alcohol for 1 min, 1% sodium hypochlorite for 10 min, T2 (Protocol 2): 70% alcohol for 30 s, 1% sodium hypochlorite for 5 min, T3 (Protocol 3): 70% alcohol for 30 s, 1% sodium hypochlorite for 5 min, T4 (Protocol 4): 70% alcohol for 30 s, 1% sodium hypochlorite for 5 min. T2 (Protocol 2): 70% alcohol for 30 s, 1% sodium hypochlorite for 5 min. T3 (Protocol 3): 70% alcohol for 3 min, 1% sodium hypochlorite for 15 min. After disinfection, nodal segments were placed in test tubes with standard culture medium (pH 5.5) containing hormones and vitamins. After 45 days, buds sprouted from the nodal segments were used for multiplication. Standard culture media were prepared with three different concentrations of hormones and vitamins: T1 (protocol 1) pantothenate 0.6ppm, IBA 0.3ppm, BAP 1ppm, GA3 1ppm, T2 (protocol 2) pantothenate 1ppm, IBA 0.1ppm, BAP 0.5ppm, GA3 0.5ppm, T3 (protocol 3) pantothenate 0.2ppm, IBA 0.5ppm, BAP 1.5ppm, GA3 1.5ppm. The results obtained showed that T1 (protocol 1) had a lower level of oxidation at 32 days with a mean of 1.36 and the highest heights of the first multiplied buds was T1 (protocol 1) with a mean of 0.6. It is concluded that T1 (protocol 1) is the most suitable for the in vitro establishment of mulberry and had the greatest effect on explant development. It is recommended to use protocol 1 for in vitro establishment of mulberry from nodal segments.