Efecto antagónico de bacterias ácido lácticas aisladas del suelo de bosque primario Cashca Totoras.

Abstract

The objective of this study was to evaluate the antagonistic effect of lactic acid bacteria (Lentilactobacillus parakefiri; Lactobacillus malefermentans and the mixed culture) isolated from the soil of Cashca Totoras Primary Forest against pathogenic agents (Salmonella spp, Staphylococcus aureus and Listeria monocytogenes) that were isolated from different food substrates according to established regulations, and subsequently used for microbial antagonism in vitro seeding with the whole milk substrate "VITA", using 0. 9% of substrate and 0.1% of bacteria to be evaluated, with a neutral medium for the growth of lactic acid bacteria and pathogens, for the collection of data was executed by using a completely randomized design, the significance between the different treatments was determined, by means of the Tukey method of separation of means (P>0.05) it is established that the LMS strain (Lactobacillus malefermentans) presents a high inhibition against Salmonella spp at 96 hours of evaluation in relation to the LPS (Lentilactobacillus parakefiri) and MS (mixed culture) strains that presented low inhibitions in the course of evaluation time, in the antagonistic capacity of the LPS strains (Lentilactobacillus parakefiri) it obtained a better inhibition against S. aureus in comparison to the strains LME (Lactobacillus malefermentans) and ME (mixed culture) and related to the mixed culture the strain with the best performance was the ML (mixed culture) presenting a better inhibition depending on the treatments LML (Lactobacillus malefermentans) and LPL (Lentilactobacillus parakefiri). Therefore, it is concluded that the use of the Lactobacillus malefermentans strain treatment is suitable for the complete inhibition of Salmonella spp. Therefore, it is recommended to use different substrates to determine the antagonistic effect using beneficial bacteria from primary forests against different pathogens.