Optimización del humedal de la planta de tratamiento de lixiviados de Baños de Agua Santa aplicando bacterias rizosféricas de Typha dominguensis para remover la demanda química y bioquímica de oxígeno.

Abstract

In this research, experiments were carried out with microbial consortia obtained from the rhizosphere of the Typha dominguensis plant from both the leachate (1) and the Typha sediment (S1) from the wetland, from the water (2) and sediment (S2) of the Laguna de Colta, and from the water (3) and sediment (S3) of the Lagoon of Langos. From samples 1, 2, 3, S1, S2 and S3, 5 dilutions of 10-1 were prepared to 10-5, these were inoculated by adding 0.5 mL of each dilution in McConkey Agar (AMC), Agar of potato dextrose (PDA) and Nutrient Agar (AN) that were incubated at 15 ºC for 24 hours. Bioaugmented microorganisms with different morphological characteristics in AMC and PDA (the microorganisms in AN were not used). The bioaugmented samples disintegrated forming microbial consortia that were part of treatments T1, T2, T3, T4, T5, T6, and T7. These will be put in contact with the leachate where 2 mL of consortium was added to 200 mL of leachate from according to each treatment, in which 600 mL was obtained by date of analysis of the 2 parameters at 15, 30 and 45 days. Using the analytical methods APHA 5220-D and APHA 5210-B, their results and by the ANOVA hypothesis test with 2 factors of mixed design taking as factors at 7 treatments and the 3 elapsed times of contact of the microbial consortium with the leachate. It was found that the most efficient microbial consortium in COD removal is T2 with 87.78% and for BOD5, T4 with 80.49%.