Inseminación artificial en aves de riña (Gallus gallus domesticus) como una alternativa para la conservación de líneas de alto valor genético.

Abstract

The outcome of the study was to use artificial insemination in fighting birds (Gallus gallus domesticus) as an alternative for the line conservations of high genetic value. The work was carried out in Orellana Province with a period of 150 days. A randomized complete block design was used, the factors under study were roosters (blind, overweight and trimmed) and hens (10 - 14; 15 - 19 and 20 - 24 months), using 2 repetitions. Evaluations of ejaculate volume, sperm concentration, spermatozoa per ejaculate, eggs number, fertile eggs, hatched eggs, live-born chicks, percentage of hatchability and fertility were performed. For statistical analysis, normality tests were tested, parametric statistics and separation of means (ANOVA-TUKEY) were applied to the data with normality, non-parametric tests of simple classification (Kruskal-Wallis) were practiced to the data without normality. It was determined that there are no differences between ejaculate volumes, sperm morphometry (with an average of 84.66% normal sperm and 15.3% sperm abnormalities; an average mass motility of 4, progressive motility of 90.66%, non-progressive motility of 6.33 %, and 3% immotile spermatozoa), production of the number of eggs, fertile eggs, hatched eggs, live hatched chicks and fertility index. The hatchability percentage showed differences between the treatments, with 84.9% (±8.6) for T1, 84.9% (±3.7) for T3, 84.12% (±4.8) for T2; identifying that the hens of T1 conferred a higher percentage. Concluding the age of the hens influences the hatchability processes, it can also be determined that in this researching the roosters age did not influence the production and characteristics of the semen. It is recommended to carry out a good handling, stimulation and extraction of the semen of the roosters.