Evaluación del efecto antibiótico in-vitro del extracto hidroalcohólico de propóleo de abejas (EHAPA) sobre bacterias ATCC causantes de Metritis Bovina.

Abstract

The Aim was to evaluate the in-vitro antibiotic effect of Apicultural Propolis Hydro-Alcoholic Extractor (EHAPA) on ATCC® bacteria causing bovine metritis, using two types of bacteria Staphylococcus aureus subsp. Aureus and Escherichia coli, divided into five treatments: T0= control treatment; T1= (15%) EHAPA; T2= (30%) EHAPA; T3= (45%) EHAPA; T4= (60%) EHAPA; with 3 repetitions for each treatment. In addition, two treatments for commercial antibiotics were included to measure Sensitivity: T5= Oxy-Tetracycline® and T6= Penicillin®. A Completely Randomised Design was applied and means were separated by Duncan's test (p ≤0.05 and p ≤ 0.01); Pearson's correlation analysis was applied for the variables studied, using INFOSTAT version 2019. In the extraction of the active principles of bee propolis, it was found that the antibiotic effect of the alcoholic extract is due to its main active principle of EHAPA, which are the partial phenols that when combined, 39.51 mg/ml were obtained. A better antibacterial response was found with a concentration of 60%, giving similar bacterial inhibition results in Staphylococcus aureus and Escherichia coli, demonstrating that as the concentration increases, the bacterial load decreases. In terms of sensitivity, its greatest action was presented at 45% and 60%, presenting a Minimum Inhibitory Concentration greater than 80%, thus differing from commercial antibiotics which presented different levels of sensitivity. It is possible to assume a superiority due to the fact that no resistance levels were found, thus concluding that the use this type of extract under these conditions presents excellent results.