Incidencia de mastitis bovina subclínica mediante la prueba de california mastitis test (CMT) con identificación del agente etiológico.

Abstract

The aim of this research work was to evaluate the incidence of subclinical bovine mastitis through the use of the California Mastitis Test (CMT) in the farm Tasinteo located in Píllaro. The identification of etiological agents and antibiograms were performed in the laboratory. Three CMT samples were taken from 34 animals in production with a total of 102 samples of which 8 were selected with positive results to be sent and analyzed in the ANIMALAB laboratory in the city of Machachi. The data were organized and analyzed to determine percentages and frequencies according to the different variables; in addition, the laboratory results were organized and analyzed to determine the quality of milk Colony Forming Unit (CFU) and sensitivity of macro-organisms to different antibiotics (antibiogram). It was determined that 24% of the samples showed a positive result for subclinical mastitis by the CMT method and 76% showed a negative result. By counting the (CFU) in the eight samples analyzed, microorganisms corresponding to Candida, Streptococcus agalactiae, Escherichia coli and Staphylococcus were found, of which Escherichia coli and Staphylococcus were the most predominant in the samples analyzed. The results of the antibiogram determined that Cephalexin showed sensitivity for most of the microorganisms found; Cefadroxil reported medium sensitivity, while with Rifampin most of the microbial agents showed resistance. It is recommended to evaluate the milking routine of the animals to determine the most predisposing factors in the appearance of subclinical mastitis in cattle.