Modificación de la membrana espermática a través de la adición de metil -β- ciclodextrina cargada de colesterol en medios de crioconservación para bovinos lecheros.

Abstract

The objective of this study was to evaluate post-thaw sperm quality in the face of the effect of addition of cholesterol-loaded cyclodextrin (CLC) in the cryopreservation medium. The research was carried out in the Animal Reproduction Laboratory at the animal Sciences Faculty at ESPOCH, using a single 3-year-old Jersey breeder from the "San Diego" Livestock, an artificial vagina was used for a total of 5 collections, macroscopic characteristics were: (color: whitish with a slightly creamy appearance, volume: 8ml, pH: 6.78) and microscopic (concentration: 1040x106 spz/ml, mass motility: 4 points, individual motility: 87% and normal morphology: 87%). The pre-diluted semen was divided into 3 equal parts adding the CLC for their respective treatments (T0:0 mg, T1:1.5 mg, T2:3.0 mg) which were incubated at 37ºC for 15 minutes, after this time the final dilution was carried out by packing them immediately into 0.5 ml straws, taking them to the automatic freezer, lowering the temperature progressively down to -196ºC and storing them in the liquid nitrogen tank, the straws were thawed every 7 days for their respective evaluation. A Complete Design Was Used Randomly obtaining significant differences P(<0.01) for motility, vitality and integrity of the membrane with means of 63.50%; 75.36%; 60.08% respectively belonging to T1, in terms of morphoanomalies no statistical differences were found, being T2 the one that presented the greatest percentage with an average of 5.40%; where the best level of use was T1, recommending the addition of 1.5 mg CLC in cryopreservation media according to the results obtained better reproductive parameters are expected.