Estudio de radiación láser de microorganismos en sistemas biomoleculares mediante la técnica de pinza óptica en el Laboratorio de la Facultad de Ciencias- ESPOCH

Abstract

The objective of the present Integrator Project was to study the effect of laser radiation from microorganisms on biomolecular systems using the optical tweezers technique in the Optics Laboratory of the Faculty of Science of the Escuela Superior Politécnica de Chimborazo. To this end, we proceeded to assemble the optical tweezers, recognising, and describing the different elements that make it up, with emphasis on the focusing and observation section. We used 633 and 650 nanometre lasers located in the near infrared so as not to disturb the sample. When the optical tweezer system was correctly calibrated, we proceeded to study the use of the ThorCam software connected to the CCD (Charge Coupled Device) camera. Samples of biomolecular systems such as urine and bovine gamete were taken, and the Optical Ray Regime was identified to perform the optical capture in the different samples. As a result, weak trapping was obtained for the 633-nanometre laser for silica microspheres and the different biomolecular systems, and with the 650-nanometre laser a much more stable trapping was obtained in all systems due to its potential, which, at the target output, was higher compared to the first laser. It is concluded that the optical trapping of microorganisms is a viable system that can be developed in the laboratories of the institution, making use of a suitable laser. It is recommended to use a laser with a power greater than 100 milliwatts to obtain favourable results.