Evaluación del antagonismo y del potencial de control biológico de hongos endófitos obtenidos de especies forestales nativas frente a Fusarium circinatum.

Abstract

The objective of this study was to evaluate the antagonism and biological control potential of endophytic fungi of native forest species against Fusarium circinatum, for which endophytic fungi previously preserved by the Castellani method were used, this research had two phases, a laboratory phase which consisted on reactivating the antagonistic and pathogenic fungi to obtain pure colonies, yto carry out confrontations between the two. Seven strains of endophytic fungi and two strains of F. circinatumwere studied and mycelial growth was measured every 24 hours with a digital calibrator aid, for both, the pathogenic fungus and the antagonist. The mycelial growth rate information was collected and the fungi were chosen according to the percentage of inhibition. In the nursery, 2- month-old P. radiata plants and 1-month-old P. patula plants were used to carry out the test with the selected strains, 576 plants were used with the treatments H329, P1R1R3, M3, M5, F. circinatumand two control treatments. With the inoculation method, after thirty days the wilting of the plants inoculated with F. circinatumbegan, the treatment with the highest incidence in P. radiata was T1 with 100% incidence and the lowest incidence was T8 with 0%, concluding that the in vitro treatments showed antagonism, but in vivo tests did not show promising results, therefore it is recommended to evaluate the effect of endophytic fungi used in this study with other pathogens of forest species.