Caracterización de bacterias asociadas a la rizósfera de Vaccinium floribundum en el páramo de Ganquis provincia de Chimborazo.

Abstract

In this research, the characterization of bacteria associated with the rhizosphere of Mortiño (Vaccinium floribundum) from the Ganquis paramo, Chimborazo province, was carried out. The procedure began with the collection of three biological samples of soil from the rhizosphere of mortiño and storing them in labeled bags in a cooler. Taxonomic profiling analysis by massively parallel sequencing of the 16S region for Bacteria began with the extraction and purification of bacterial DNA, taking 500gr of soil from each sample and using the FastDNA™ SPIN Kit for Soil instrument, the purified genetic material was extracted. The quality analysis of the extracted genetic material was then carried out by amplification of the 16S RNA gene. In the elaboration of the databases, the readings obtained were cleaned and assembled using bioinformatics software, eliminating chimeric sequences, and improving the quality of the results. The results of this bioinformatics process yielded 488 bacterial operational taxonomic units (OTU's), which were taxonomically organized from phylum to species. According to the classification criteria, beneficial and pathogenic bacteria for plants, bacteria of undefined function, and bacteria of other functions not related to plants were identified. It was classified 9 bacterial phyla, of which the most abundant is the phylum Proteobacteria, comprising 219 OTU's, corresponding to 45% of the total number of OTU's identified. It was concluded that of the 488 OTU's identified, 196 OTU's of beneficial activity, 6 OTU's of pathogenic activity, 232 OTU's of undefined function, and 54 OTU's of non-plant- related functions were found. It is recommended to carry out a metagenome and meta transcriptome sequencing to verify the suggested functionalities of the bacterial OTU's in this study.