Sensibilidad in vitro de Fusarium spp. asociado a muerte de plantulas de Pinus radiata y Pinus patula a fungicidas de diferente modo de acción.

Abstract

The aim of this study was to determine the in vitro sensitivity of Fusarium spp associated with death of Pinus radiata and Pinus patula seedlings in nursery to fungicides with different modes of action. The mycelial growth rate and the effective dose 50 (ED50) were established for the active ingredients: Fludioxonil, Hymexazol, Tiabendazole, Azoxystrobin and Fluazinam, which were used in doses of 0, 0.1, 1, 10 and 100 ppm. The mycelial growth of Fusarium spp. was measured every 24 h during 15 consecutive days to obtain the variables to be evaluated in two hosts. The results showed that Fusarium spp. was very sensitive according to the Edgington scale to Fludioxonil as active ingredient, since it generated the greatest inhibitory effect on mycelial growth with the 1 ppm concentration compared to the other products used in this study, It also reached the lowest effective dose 50 (ED50) with an average of 0.013 mg/L with isolate 4 of Fusarium spp. while Azoxystrobin reached the designation of resistant according to the Edgington sensitivity scale, with an average of 263.80 mg/L for the second isolate of Fusarium spp. Therefore, the use of Fludioxonil in nurseries is recommended as a convenient alternative for the control of Fusarium spp. It is also recommended to carry out sensitivity studies different from the active principles of this study for the control of fungi causing diseases in forest species.