Evaluación de la actividad anti-fúngica y anti- bacterial de un extracto alcohólico y acuoso de hojas y resina de Dacryodes peruviana (copal), bajo condiciones de laboratorio.

Abstract

This research aimed to evaluate the antifungal and antibacterial activity of the alcoholic and aqueous extract of the leaves and resin of Dacryodes peruviana as controller Ralstonia solanacearum and Fusarium sp. The leaves and resin of Dacryodes peruviana were identified, collected and stored in order to get the different extracts both alcoholic and aqueous through the maceration method. This method consists of the solid-liquid. To do this, the leaves and crushed resin were used in order to obtain the two extracts that were tested at the laboratory level. Different culture mediums were carried out for the activation of the bacteria and the fungus such as Nutritive Agar for the first one and Potato Dextrose Agar for the second one, which were covered them up. Once the two microorganisms were activated, the determination of the antifungal and antibacterial potential were performed. Once the medium culture was inoculated with the bacteria and the fungus, the aqueous extracts were inoculated and Dacyodes peruviana alcoholics at different concentrations that were 50%, 35% and 25% for the fungus and the bacteria, the direct inoculation methodology was used, placing 4 drops of 25 μL of the alcoholic and aqueous extract, an experimental design of the Complete Design type was implemented at the random with five repetitions for each experiment. In each treatment the halos of inhibition at 120 h. with significant differences between the treatments, the efficiency of the alcoholic extract was higher than that of the aqueous extract for both Fusarium sp and Ralstonia solanacearum. It was concluded that there was antifungal and antibacterial potential in excerpts, providing a guideline for further research.