Determinación de Buenas Prácticas de Producción de Ratas (Rattus norvegicus) en el Bioterio de la Escuela de Bioquímica y Farmacia. [Incluye Manual de Uso y Ciudado de Animales de Experimentación].

Abstract

"The Biochemisty and Pharmacy School at the Escuela Superior Politécnica de Chimborazo have an animal facity cested wi the purpose o improve the leaming- teaching process trough he research. Using latanimals with ntematinal standard, “The cbjecive o rs research sto detormino parameters of good production practicas (PSP) of Rattus norvegicus Irough Iho physical and microbilogial test. Bath experimental animals and is environment. Microenvionmental factors were analyzed such as: jals, fosders, food and water, and macroenvionmental such as ai, omperaturo, humidiy, It intensity and noise, during Novembor 2009 — Apei 2010. “The microbilogical analysis showed the air exceeds the perited rango of minus SUFGIm? of mesophiic aerobes, who tho temperatura physical analysis, humidty, light intensty and noise, explain ho parameters full with tho specified requirements for the National Normalizaton Off from Cuba for macroenviormental factors. The icrbiolgical analysis of the microenvicemental factors show the containing Microtial in jas, fesders and shelves exceede the permited range of minus SUFCIM? dl mesophlc aercbes, inicaing ho food in the loss for enerobacteriacass, total alforms, mouldyeast and E. cal show a content Very Numerous Bscteria o Count (VNEC); also shows the water does not full vit the specified requirements in mesophlle aerobes and total colors, however compl with the fecabcolrm absence requiremnt according 1o INEN Standard 1105. The Iaboratoy-animals icrbiolgical analysis showed; 20% ol tha animals have ectoparaites and 40% endoparasites such as Giarda Jambia cysis 30%, Estronglides estercorals larvas 10%, Enterobius vermicuaris eggs 10% and Trchuis trchiura eggs 10%. The pharyngeal secrtion analysis and cecum content show 100% of he animals have al loas! type ol micoorganism such, Candida alicans 50%, Escherichia col 80%, non- group A Steplococcus 30%, Enterobacter gergovias 10%, Proteus miablis 10%, Enterobacter cloacas 20% and Yersnia enterocolica 20%. The POP Rattus noregicus determination, shows the management and persomel care in each production and maintenance area should bo improved folouing the estabished parameters for ntematonal organizations."