Determinar la eficacia de los métodos, Elisa en suero sanguíneo y la detección cualitativa del antígeno de Helicobacter pylori en heces fecales en los estudiantes de bioquímica y farmacia – ESPOCH

Abstract

The purpose of this work was to compare the efficacy of the Elisa methods in blood serum and the qualitative detection of Helicobacter pylori antigen in feces in Biochemistry and Pharmacy students of the Polytechnic School of Chimborazo, through a non-experimental study, descriptive, cross-sectional, made up of 92 students and teachers, between an age range of 19 to 50 years, who met the inclusion criteria. The analysis was carried out in the ESPOCH Clinical Laboratory, complying with the biosafety and quality control standards. A data collection sheet was designed using Microsoft Excel and SPSS statistical software for tabulation and analysis. In the results, it was observed that 67.39% of the students obtained positive results and 32.61% negative, by the Elisa method in blood serum; on the other hand, 18.47% were positive, with 8.69% negative by qualitative detection of helicobacters pylori antigen in feces. It was determined that, of the positive cases, 84.8% wash fruits and vegetables with tap water, 56.5% consume food sold by street vendors. According to the symptoms, 19.6% present abdomen swelling, with 14.2% has heartburn, 10.6% cruet and other stomach upset. Based on this analysis. It is recommended that students from the School of Biochemistry and Pharmacy encourage talks in the students' homes about the causes and consequences produced by the bacteria in order to contract cancer-causing diseases in the stomach.