Efecto de la temperatura y pH sobre el proceso industrial para la obtención de bioetanol por hidrólisis enzimática a partir de Colocasia esculenta (Papa China).

Abstract

The objective of the present investigation was to evaluate the effect of temperature and pH on the industrial process for obtaining bioethanol by enzymatic hydrolysis from Colocasia esculenta (chinese potato). The bromatological characterization was carried out following the methods described by the Association of Official Analytical Chemists (AOAC, 1990), where a humidity percentage of 81%, protein of 5.84%, starch 12.44%, fat 1.3%, fiber 2.1% and ash were obtained. 3.2%., To evaluate the variables under study in the saccharification and distillation processes, three temperatures were evaluated: 40, 60, and 80 ° C and three pH: 4, 6 and 8. In the saccharification process it was obtained that with the treatment T2H2 (60 0C, pH 6), and T1H2 (40 0C and pH 6), they presented the highest amount of brix degrees with values of 16.033 and 16.00% respectively, which allowed to deduce that at these temperatures higher conversion is obtained. In the distillation stage the treatments T1H1 (40 0C, pH 4), and T1H2 (60 0C, pH 4) presented the highest volume of distillate (53 and 47 ml) respectively. The brix degree values of the distillate are lower in the T1H2 treatment, inferring that the saccharification process was effective since the brix degrees were reduced from 16 to 0.999 percent, concluding that to obtain a greater volume of bioethanol it is advisable to use temperatures between 40 and 60 0C and acidic pH between 4 and 6. Of the variables analyzed, it is recommended that for the saccharification stage with glucoamylase in obtaining bioethanol from C. esculenta by enzymatic hydrolysis, use temperatures of 550C, and an acid pH of 5 units.