Análisis microbiológico y resistencia bacteriana en el ceviche de chocho (Lupinus mutabilis sweet) y su impacto en la salud pública de Riobamba – 2019

Abstract

This degree work main aim was to develop a microbiological analysis of the chochos ceviche using traditional cereal grains as a feedstock expended in the places of greatest concurrence, Riobamba City-Ecuador to evaluate the microbiological quality and bacterial resistance in this food for mass consumption to determine whether or not it is suitable of your food intake. Also, it seeks to demonstrate the hygienic-sanitary conditions in which it is processed and marketed; frame in the reference regulations: NTE INEN 2390: Bittering process of the cereal and the Sanitary Standard for food and beverages for human consumption - Peru. Article 15, section XV. The study was carried out in 7 retail outlets registered in the GAD-Riobamba: Teodoro Gallegos Borja coliseum and plaza of the neighborhood of Villa María in the period running from October to December 2019. Compliance with the health assessment guide for retail outlets was verified Prepared food outpatients, samples were taken for analysis in the morning and the afternoon to observe the variability in microbiological quality, the dilutions tested were 10^(-4) y 10^(-5). Mesophilic aerobes, total coliforms, Escherichia coli, Staphylococcus aureus, Salmonella, parasites, molds, and yeasts were quantified and qualified by conventional culture methods, including the isolation and identification of pathogenic bacteria by molecular techniques. As a result, a deficit in the hygienic-sanitary quality was evidenced, finding values higher than the permissible microbiological limits, establishing that this food can represent a source of ETA's infection. Most of the identified bacteria showed resistance to drugs such as Ampicillin and Amoxicillin + clavulanic acid. Sanitary control entities should periodically verify the application on good hygiene practices in food handling and training vendors.